Wasps back Paolo Odogwu and Bristol scrum-half Harry Randall have won their first England call-ups for the Six Nations.
Bath prop Beno Obano is the third uncapped player to be named by Eddie Jones in the 28-man squad.
Courtney Lawes returns from injury, while Mako Vunipola, who last played in the Autumn Nations Cup final win over France, will join the squad for rehabilitation but be unavailable for selection.
Wasps flanker Jack Willis and Bath's Jonathan Joseph only make the 12-man shadow squad, while Kyle Sinckler, who is banned for the opening game against Scotland on February 6, is not in either group.
Owen Farrell will retain his position as captain.
The squad will meet up at St. George's Park on January 27, with coach Jones, who is isolating after assistant Matt Proudfoot tested positive for coronavirus, set to arrive a day later.
"This has been a really tough squad to pick and I know there are a lot of disappointed players who haven't been selected," said Jones.
"We're really excited by the players we have got, it's a very vibrant squad and are looking forward to getting started next week.
"We're very grateful to the Premiership Rugby, the clubs and RPA [Rugby Players Association] for allowing us to have 28 players throughout the tournament, we're very happy with the number and it's testament to the growing relationships between all parties.
"Ultimately our goal is to win the Six Nations and to do that we'll need to work hard and prepare well each day and take it as it comes."
England squad:
Forwards: Luke Cowan-Dickie, Tom Curry, Ben Earl, Ellis Genge, Jamie George, Jonny Hill, Maro Itoje, Joe Launchbury, Courtney Lawes, Joe Marler, Beno Obano, Will Stuart, Sam Underhill, Billy Vunipola, Harry Williams, Mark Wilson.
Backs: Elliot Daly, Owen Farrell, George Ford, Ollie Lawrence, Max Malins, Jonny May, Paolo Odogwu, Harry Randall, Dan Robson, Henry Slade, Anthony Watson, Ben Youngs.
Shadow squad: Charlie Atkinson, Ali Crossdale, Tom Dunn, Charlie Ewels, George Furbank, Joe Heyes, Jonathan Joseph, Joe Marchant, George Martin, Alex Mitchell, Jacob Umaga, Jack Willis.
Carsley: No more 'copy and paste' England squads
Interim England boss Lee Carsley says he will not "copy and paste" his squads, with players to be rewarded for strong club form with Three Lions call-ups.
Carsley oversaw 2-0 Nations League victories over the Republic of Ireland and Finland last month, having taken the reins on a temporary basis after Gareth Southgate's resignation.
The former under-21 boss announced his latest 25-man squad for upcoming matches against Greece and Finland on Thursday, making four changes from last month's party.
Tottenham striker Dominic Solanke won his first call-up in seven years, with Eberechi Eze, Harry Maguire, Tino Livramento and Jarrod Bowen all left out.
Predecessor Southgate was often criticised for sticking with a trusted core of players during his eight-year spell with the Three Lions, but Carsley says nobody is guaranteed a place under him.
"In the back of my mind, I wanted to make sure it feels fresh," Carsley said.
"When you get that email or WhatsApp to say that you've been called up, it's an achievement because it shouldn't be something that is taken for granted.
"I thought it was important that it was not just a copy and paste. I want them to feel like they have earned their place in the squad."
Tottenham midfielder James Maddison and Manchester United forward Marcus Rashford – both of whom were left out of Southgate's Euro 2024 squad – were among the players to be overlooked.
Carsley said he had been left with several tough decisions to make but had prioritised those in form in order to build rhythm before the 2026 World Cup qualifiers start next year.
"The players I've left out, I could make a case for them being in, but ultimately I have to make those decisions," Carsley said.
"The reality is you want a player to be coming in in top form and fighting for a position in the team.
"Getting out of this Nations League is important for us in terms of setting up World Cup qualification. Whether I'm here or not, it's important we are in a favourable pattern and in a position to win the World Cup."
Lee Carsley insists Harry Maguire's England omission not due to form
Lee Carsley insists Harry Maguire's omission from his latest England squad is not due to form and he still has an important role to play for his country.
Manchester United centre-back Maguire missed Euro 2024 for beaten finalists England due to injury but was involved for interim boss Carlsey's first squad since Gareth Southgate departed for the Nations League victories over the Republic of Ireland and Finland last month.
However, there was no place for the 31-year-old ahead of this month's Nations League double-header against Greece and the return clash with the Finnish.
Maguire was the fifth-most utilised player for England under Southgate, with only Harry Kane, Kyle Walker, Jordan Pickford and John Stones appearing more, and Carsley explained that the decision was just to take a look at other options.
"It was a chance of having a look at other players. It's nothing to do with form. It's looking at the next two camps to look at other players," Carsley said.
"The players left out all have a case but we saw the benefit in the last camp of the squad being fresh, feeling fresh and a lot of energy in it.
"It's not just a copy and a paste, I want them to feel like they all have earned their place in the squad. People want to know where they stand long-term, I've told all the players this is a short-term situation.
"Harry is a player I've worked with in the past. He was very positive in the conversation we have.
"It was a case of talking to him about what my decision was, we have some really good centre-backs who we are keen to see play. Harry still has a really important part to play, not just in this squad but also in the campaign."
Gillespie defends under-fire Pakistan captain Masood ahead of England Test series
Jason Gillespie insists Shan Masood has a "clear plan" as Pakistan's Test captain.
Pakistan's red-ball skipper has faced scrutiny after overseeing five straight defeats as captain, starting by being whitewashed 3-0 by Australia last December.
That was followed by a shock 2-0 series defeat to Bangladesh last month, which marked Gillespie's first as Pakistan Test coach.
Next up is a three-match series with England, where Joe Root (12,402) is 71 away from surpassing Alastair Cook (12,472) as the all-time leading run scorer for England in men’s Tests, beginning in Multan on Monday.
Speaking on BBC World Service's Stumped podcast, Gillespie said of Masood: "Shan has got a very clear plan on how the Test side should play and we are completely aligned on that," the 49-year-old said.
"There are things people don't see and I've been really impressed by his communication with the players and how he's gone about doing his job."
Gillespie added that he believes part of the issue is improving the fitness of the team.
He added: "We can't afford to have players in international cricket who are not fit or strong enough to negotiate the rigours of the game.
"What I've learned from the Bangladesh series is that we've got some wonderful players, but we need to be more consistent. We've got the skills but we need to keep fine-tuning them.
"There's also been plenty of talks around fitness and we're making sure that it's a key component for us going forward."
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