Ash Barty crashed out of the Adelaide International at her first hurdle after going down in straight sets to Danielle Collins.
It took only 65 minutes for American Collins to defeat the defending champion and world number one in a 6-3 6-4 triumph.
Barty, who had a bye in the first round, did have opportunities, breaking in the first game of the match and building a 4-1 lead in the second set.
But on both occasions Collins stormed back, claiming her first win in four attempts against home favourite Barty, who had beaten her in the semi-finals of last year's tournament.
Coco Gauff continued her fine run to reach a first WTA quarter-final since August 2020 with an impressive comeback win over sixth seed Petra Martic.
The American triumphed 5-7 6-3 6-4 in two hours and 12 minutes to book a clash with compatriot Shelby Rogers at the WTA 500 event.
Gauff did not relinquish serve once after losing the first set and did not even offer up a break-point chance in the decider, which she sealed with a pivotal early break secured with a passing winner.
"I go into every tournament thinking that I can win," said the 16-year-old.
"Obviously that's impossible, to have a perfect record, but I go into every tournament believing that I can win.
"I try not to think far ahead in the match, at least I just try to focus on my next match, just because you have to get through that before you can get to the finals, and just take it one match at a time."
Second seed Belinda Bencic will face Storm Sanders in the quarter-finals after a 6-1 6-3 win over Misaki Doi.
In a match where she was not quite as dominant as the scoreline suggests, Bencic had to fend off 12 break points but was successful in saving 10 of them.
Number four seed Elise Mertens withdrew before her match with a shoulder injury, so Anastasija Sevastova beat lucky loser Christina McHale to set up a last-eight tie with Jil Teichmann.
Djokovic thrilled by prospect of 'one last dance' with Nadal
Novak Djokovic is thrilled by the prospect of meeting Rafael Nadal for "one last dance" at the Paris Olympics, if the Spaniard overcomes an injury setback to feature at Roland-Garros.
Thursday's men's singles draw saw Djokovic paired with Australia's Matthew Ebden in the first round, with Nadal taking on Marton Fucsovics.
The legendary duo – who have won 46 grand slam singles titles between them – will meet in the second round if they both advance through their openers this weekend.
Djokovic and Nadal have previously faced off on 59 occasions, with the Serbian winning 30 of those contests but losing four of the last six, the most recent being a Nadal victory in four sets in the 2022 French Open quarter-finals.
Nadal's participation at the Games was thrown into doubt when his coach Carlos Moya said he had suffered a "setback" on Thursday and required 48 hours of rest.
If Nadal – who is widely expected to retire later this year – does make the tournament, Djokovic is looking forward to the prospect of facing him for a 60th time.
"Obviously he hasn't played much. His ranking dropped, so there was always going to be that possibility of me meeting him in an early round and there we go," Djokovic said.
"It's going to be a spectacle, definitely, if we get to meet. We will spread some fireworks on the court, like the good old times.
"I hope we get to meet because it will probably be one last dance for the both of us."
Djokovic is set to appear at his fifth Olympics in the French capital, the joint-most by any male player since the sport returned to the Games in 1988 (with Yen Hsun Lu).
He is the player with the most matches played (19) and joint-most matches won (13, level with Roger Federer) at the Olympics since 1988.
However, his best result at the Games remains a Bronze medal at Beijing 2008, when he was beaten by eventual gold medallist Nadal in the semi-finals.
Djokovic lost the bronze medal match to Pablo Carreno Busta at the delayed Tokyo Games three years ago, also losing to Juan Martin del Potro at both London 2012 and Rio 2016.
Linette dominates first all-Polish WTA final to win Prague Open
Magda Linette rounded off her Olympic preparations in style as she claimed her third WTA title with a comfortable win over Magdalena Frech at the Prague Open.
Linette, who beat Viktoriya Tomova and Linda Noskova on her way to the final, won in straight sets 6-2 6-1 as she now heads to Paris to face Mirra Andreeva.
The world number 41 was in the ascendency from the off, forcing a break in the fourth game and never looking back.
Despite the best efforts of Frech, who saved one set point, Linette continued to apply the pressure and another break put her a set up.
Linette would again break in the fourth game of the second set, winning three games on the bounce to secure her first career win over her compatriot.
Data Debrief: Linette prevails in first all-Polish affair
The final in Prague between Linette and Frech was the first all-Polish WTA level final in the Open Era.
Linette's triumph over her opponent was her first, having lost in the pair's previous encounter in Saint-Malo back in 2022.
During the contest, the world number 41 gave nothing away, saving all four of the break points she faced while converting three of the seven she had against Frech.
Nadal suffers injury 'setback' as Olympics participation thrown into doubt
Rafael Nadal may be facing another injury setback at the Paris Olympic Games after his coach warned over the Spaniard's fitness.
The 22-time major champion is widely expected to retire at the end of this year, though is preparing for a final swansong at Roland-Garros, where he has won 14 titles.
Nadal is set to play in the singles and doubles tournaments of the Olympics, partnering current French Open and Wimbledon champion Carlos Alcaraz for the latter competition.
Yet his participation has become doubtful after coach Carlos Moya confirmed Nadal had cancelled his training session on Thursday due to a hamstring issue.
"It was a setback and we decided that today Rafa would rest and not train to recover," Moya said, as quoted by Spanish journalist Rafael Plaza.
"We are going to give ourselves 48 hours, we have to wait."
The 38-year-old is reportedly feeling soreness in both his left and right hamstring having pulled out of a scheduled practice session with Alexander Zverev.
Alcaraz had been partnering Nadal throughout the week during training on the clay courts of Paris, though the veteran's feature has now been thrown under a cloud of doubt.
Nadal is one of just two players to have won Olympic gold in both singles and doubles since the sport returned to the Games in 1988, doing so at Beijing 2008 (singles) and Rio 2016 (doubles).
In the singles in the French capital, Nadal has been drawn against Hungary's Marton Fucsovics in the opening round, and the prize could be a second-round date with long-term rival Novak Djokovic.
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