Luka Modric could miss Real Madrid's Copa del Rey final clash with Osasuna and the first leg of the Champions League sem-final against Manchester City after suffering a thigh injury.
Modric sustained an injury to his left leg during Madrid's surprise 4-2 defeat at Girona on Tuesday, a result which kept them 11 points adrift of LaLiga leaders Barcelona.
The blow comes at an unfortunate time for Los Blancos, who will bid to end a nine-year wait to lift the Copa del Rey on May 6, before hosting City three days later.
Asked about Modric's fitness ahead of Saturday's fixture against Almeria, Ancelotti acknowledged losing the Croatian's experience would be a big setback.
"He got an injury in the game against Girona. We will see how he progresses in the next few days," Ancelotti said.
"He will be doubtful for the Copa final. We must evaluate him, we are hurt, but it can happen. I hope he recovers for the important matches.
"We have very important midfielders like Federico Valverde, Eduardo Camavinga and Aurelien Tchouameni. We have many resources.
"But to replace Luka… you cannot replace his experience in these kinds of games."
Madrid have won just four of their past nine league games (D2 L3) to allow Barca to pull clear at the summit.
Pushed on why Madrid had failed to replicate their cup heroics in LaLiga this term, Ancelotti said: "I don't know, it's a matter of concentration, it's normal, not always being able to be at the top.
"The schedule is very demanding and I see many coaches complaining about this. We are human and it's the truth, sometimes I can't prepare all the games in the same way either.
"In some games you concentrate better, and in others it's worse. It happens to everyone, good days and bad ones.
"You have to trust the players. I see the group in great shape. What happened last season was unforgettable and we want to repeat it."
Ederson injury 'doesn't look good', says Guardiola
Pep Guardiola conceded Ederson's injury "doesn't look good" as Manchester City were forced to wait on the severity of the issue.
Ederson had to be taken off at half-time during City's 2-0 win over Nottingham Forest on Sunday, a victory that took them back to within a point of Premier League leaders Arsenal with a game in hand.
The Brazil international needed treatment on an apparent shoulder injury after a collision with Forest defender Willy Boly.
Ederson was seen with his arm in a sling after full-time at the City Ground.
And Guardiola said: "It doesn't look good. We will see [on Monday] with the doctor."
Stefan Ortega again stepped up in Ederson's absence, with City's number two having performed well when called upon this campaign.
Ortega has made seven Premier League appearances this season, making 16 saves from 22 shots on target faced, giving him a save percentage of 72.7.
Ederson, meanwhile, has a save percentage of 67.1, keeping out 53 of the 79 attempts on target he has faced.
Ortega has a slightly better goals prevented figure of 1.6 compared to Ederson's 1.0.
Nuno encouraged by Forest's attitude and commitment, despite Man City defeat
Nuno Espirito Santo believes Nottingham Forest will "achieve what we want" by replicating the attitude demonstrated during their defeat by Manchester City.
Forest went down 2-0 against their title-chasing opponents at the City Ground, and remain just a point clear of the relegation zone with three games remaining.
Despite the defeat, the hosts produced a spirited performance against Pep Guardiola's side, while registering 14 shots on goal compared to 11 from the Citizens - meaning they had had more shots in both meetings this season.
Forest face already relegated Sheffield United, Chelsea and fellow strugglers Burnley in their final three games, and Nuno has seen enough to be confident of securing the Reds' Premier League status for another year.
"We played a good game, were compact, organised and had chances," he told Sky Sports. "We did a good job regarding how hard it is to play against Manchester City, and the players gave it a big go.
"We are disappointed with the result, but the encouragement comes from the way we work, how committed we are, and what we have ahead of us. This is more important.
"We stay positive, and we go for the next three games. With this attitude, we will achieve what we want."
Defender Moussa Niakhate echoes his manager's thoughts, adding: "We had three big chances in the first half, but we didn't score. That was the difference.
"Everybody is working hard. We can be proud. If we perform like this in the next three games, we'll stay up."
Gvardiol: Four more finals for City in title fight
Josko Gvardiol believes there are "four more finals" for Manchester City between them and the Premier League title.
City won 2-0 at Nottingham Forest on Sunday thanks to a first-half header from Gvardiol before a 71st-minute Erling Haaland finish wrapped up the points.
The victory moves City one point behind leaders Arsenal with a game in hand. The Gunners played earlier on Sunday, winning 3-2 at north London rivals Tottenham.
The destination of the Premier League title remains in City's hands as they bid for a sixth Premier League title in seven seasons, and Gvardiol acknowledged that every game between now and the end of the season is vital.
"We need to be focused on ourselves," Gvardiol told Sky Sports. "That’s what we are doing.
"There are four more finals to the end so let's do it."
Gvardiol's goal set City on course for victory at the City Ground, nodding home from Kevin De Bruyne's inswinging corner.
It was only Gvardiol's second Premier League goal since arriving at City from RB Leipzig prior to this season.
Gvardiol was somewhat surprised to get on the scoresheet, saying: "I didn't expect to score, but I found myself in a good position.
"It was really tough especially as the pitch was really dry. It's a more than important three points."
Pep Guardiola was delighted to come away with the three points against a Forest side fighting for their lives at the bottom, with the defeat to City leaving Nuno Espirito Santo's men in a perilous position, just a point above the relegation zone with three games to play.
"It was a difficult game," Guardiola told Sky Sports. "They played with bravery. They have fast players with a lot of quality.
"It is a great victory as we had a lot of games behind us. These games are so, so tough at this stage in the Premier League. Now we have a long week to recover."
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