Serena and Venus Williams saw hopes of one last US Open glory run together scuppered by a Czech pair who apologised for knocking out the American superstars.
The Williams sisters lost 7-6 (7-5) 6-4 against Lucie Hradecka and Linda Noskova on Arthur Ashe Stadium, with the doubles match given top billing in the New York night session.
Such scheduling had previously been unheard of, but such is the attention being foisted on the sisters that first-round doubles encounter was the match everyone wanted to see, knowing it could well be the last time the siblings play as a team.
Serena has indicated, albeit couched in somewhat vague terms, that this US Open will be her farewell to tennis. Venus, now 42, may follow suit and also retire from the sport.
While 40-year-old Serena remains in the singles, having beaten world number two Anett Kontaveit in round two to set up a Friday night clash with Ajla Tomljanovic, the dream of one more Flushing Meadows doubles title is over.
It was Czech-out time for the duo who have won 14 grand slam doubles titles together, and Hradecka said sorry to the partisan crowd for spoiling the party.
"I'm still in shock that we won, because we were playing for the first time with each other and I think we did a very good job," Hradecka said.
"I'm so sorry for you that we beat them, but we are so happy that we did it."
Hradecka had been planning on playing with Indian Sania Mirza, but an injury for Mirza put paid to that idea, meaning a new partner was required.
Noskova, 17, answered the 37-year-old Hradecka's call, and enjoyed a night to remember.
"Playing against the Williams sisters is a special moment for everybody, anytime," Noskova said.
"I was really lucky I could play with my doubles partner and we could win and pull out a good fight."
It was the Williams' first doubles match at a grand slam since the 2018 French Open, and their first at the US Open since 2014, and although it was a tight contest the younger pair just had the edge.
Neither sister gave an on-court interview or a press conference after the match, with all eyes now turning towards Serena's singles campaign, and the possibility of that extending deep into the second week.
Jarry defeats Paul to set up Zverev showdown in Italian Open final
Nicolas Jarry defeated Tommy Paul in three sets on Friday to set up a showdown with Alexander Zverev in the Italian Open final.
The men's world number 24 prevailed 6-3 6-7 (3-7) 6-3 in a little under three hours in Rome to reach his first ATP Masters 1000 final.
Jarry earned the only break of serve in the eighth game of the opening set, but Paul - who dropped just one set in four matches en route to this stage - hit back in the second.
Birthday boy Paul lost serve to trail 4-2 at one stage, but he produced one of the shots of the tournament at the ideal moment and soon dragged it back to 4-4.
A gruelling set concluded with Paul taking the tie-break 7-3, but Jarry was unperturbed and won the deciding set with his fifth match point to see off Paul in a gruelling contest.
Data Debrief:
Jarry is the third male Chilean in the Open Era to reach the singles final of the Italian Open after Marcelo Rios (1997-98) and Fernando Gonzalez (2007).
The 28-year-old registered 13 aces against Paul to his opponent's seven and hit 35 winners to 19 in a deserved victory.
Zverev ends Tabilo's dream run to reach Italian Open final
Alexander Zverev fought back from the brink to reach the Italian Open final on Friday, overcoming Alejandro Tabilo by a 1-6 7-6 (7-4) 6-2 scoreline in the last four.
Zverev was punished for a slow start as Tabilo took the opener within 32 minutes, but the German fought back in a one-hour, 15-minute slog of a second set.
The 2017 Italian Open champion put his foot down from 3-3 in the second-set tie-break, with Tabilo potentially feeling the effects of a fine run that included a last-32 upset of Novak Djokovic.
Zverev upped the intensity in the final set and won a huge 94 per cent of points behind his first serve as his opponent wilted, the third seed reaching the Rome final for a third time.
Data Debrief: Zverev draws level with Becker
Zverev will now face either Nicolas Jarry or Tommy Paul in Sunday's final, which will be his 11th at ATP 1000 Masters level.
That tally pulls him level with Boris Becker for most such finals by a German player since the format's 1990 introduction.
Sabalenka defeats Collins to set up Swiatek rematch
Aryna Sabalenka defeated Danielle Collins 7-5 6-2 to set up yet another meeting with Iga Swiatek at the Italian Open.
Having defeated Collins en route to the final of the Madrid Open, which she lost to Swiatek, Sabalenka repeated the trick with a straight sets win over the American in Thursday's semi-final.
And the Belarusian's reward will be an immediate rematch with Swiatek.
The duo have met 10 times, including in Madrid earlier this month, with Swiatek winning seven times and Sabalenka claiming three victories.
This will be Sabalenka's first appearance in the Italian Open final.
She is the sixth player, along with Simona Halep (2017), Dinara Safina (2009), Serena Williams (2013), Ons Jabeur (2022), and Swiatek (2024) to have reached the final of both Madrid and Rome in the same season.
Data Debrief: Perfect record
Collins is the only player against whom Sabalenka has registered six wins without a loss in WTA events, while only against Maria Sakkari and Elise Mertens does she have more wins in her career in such events (seven each).
The final will mark the fourth meeting on clay between Swiatek and Sabalenka as world number one and two, equalling Chris Evert and Martina Navratilova for the most meetings on the surface as the WTA's top-two ranked players.
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