Mikel Arteta questioned the decision to send off David Luiz after the defender was one of two Arsenal players to be dismissed in a 2-1 defeat at Wolves.
Arsenal appeared set to go in at half-time deservedly ahead following Nicolas Pepe's opener, only for David Luiz to concede a penalty following a challenge on Willian Jose.
The Brazilian centre-back was shown a red card by referee Craig Pawson following a VAR check, though Arteta insisted he could not see any contact after studying replays of the incident.
Ruben Neves converted from the spot before Joao Moutinho's long-range strike put the hosts ahead shortly after the break, with any hopes of a fightback disappearing for Arsenal when goalkeeper Bernd Leno was given his marching orders for handling the ball outside the area.
For Arteta, though, it was the David Luiz call that left him frustrated, particularly after his side had played so well up until that crucial moment in the contest.
"First of all, I have to say that I'm proud of my team, the way we played and the way we dominated the first half," Arteta told the media.
"We should have been 3-0 or 4-0 up. We didn't do that, though, and we put ourselves in a really difficult position by conceding the goal and going a man down.
"I have seen the replay 10 times from five different angles and I cannot see any contact. I would like to see if VAR has a different angle. I'm expecting to see something and I'm not seeing anything.
"We were really animated at half-time because we really wanted to win the game. The boys were so convinced to go out there and have a real go.
"We didn't concede any chances but Moutinho scores a worldie and it's 2-1. Then, when Bernd comes out and hits the ball with his hand, we're down to nine men and almost impossible to win the game."
Since making his league debut for Arsenal in August 2019, David Luiz has picked up three red cards and conceded six penalties, more than any other player in the competition in both categories.
Arteta - who has seen his team shown nine Premier League red cards since taking charge, six more than any other top-flight team - admitted the result was tough to take.
"The way we lost the game really hurts. It's painful," he said.
"I have to take the positives as well, which is the way we played against this team that is very difficult to attack, the amount of chances we created. But we leave the ground and have zero points and have lost two important players.
"It's hard enough to win football games in this league. There's nothing we can change. It was a big decision, if they got it right and can justify they got it right, I'll put my hand up and apologise.
"The only thing I'm saying is that I’m sitting here and I cannot see any contact. That's really, really frustrating, because it's a big, big moment in the game."
Asked if Arsenal may issue an appeal against the red card for David Luiz, Arteta replied: "Where I’m standing now, I'd say yes – let's go straight away. But I don't know, we will have to speak to the legal team at the club and decide if it is the best thing."
Runar Alex Runarsson came on to take over from Leno in goal, with recent recruit Mat Ryan ruled out with a muscle issue. Arteta revealed he is hopeful the Australia international can be fit in time for Saturday's trip to Aston Villa.
Girona 0-1 Villarreal: Traore strike lifts Yellow Submarine
Bertrand Traore's goal gave Villarreal a 1-0 win at Girona on Tuesday that prevented the hosts from reclaiming second spot in LaLiga.
Girona, who have enjoyed a superb campaign, are third with 75 points, trailing Barcelona, who moved a point clear with a 2-0 home win over Real Sociedad on Monday.
Alexander Sorloth and Goncalo Guedes went close for Villarreal in the first half, but Girona goalkeeper Paulo Gazzaniga was in fine form.
Traore broke the deadlock in the 59th minute after latching on to a superb pass from Guedes, however.
Viktor Tsyhankov, Savio and Artem Dovbyk all went close for Girona, but could not find the back of Filip Jorgensen's net.
Villarreal were reduced to 10 men in the 95th minute after Kiko Femenia was sent off for a second yellow card after a push on Aleix Garcia.
Data Debrief: Rare home loss for Girona
Girona have suffered only their second defeat at Montilivi in LaLiga this season, following a 0-3 loss to Real Madrid in September.
The home side attempted 749 passes in this game, their highest total in a single match in LaLiga this season.
Postecoglou rues missed chances against City as Son squanders decisive opportunity
Ange Postecoglou was left to rue missed chances as Tottenham's Champions League hopes were ended after Tuesday's 2-0 defeat to Manchester City.
Spurs needed three points to take the Premier League's top-four race to the final day but were undone by Pep Guardiola's side, who moved within touching distance of another top-flight title.
Erling Haaland's routine tap-in from Kevin De Bruyne's brilliance after 51 minutes proved a cruel blow before the City striker's stoppage-time penalty sealed a decisive three points.
It could have been a different story, though, if Stefan Ortega – on as a second-half substitute for Ederson – did not deny Heung-min Son's gilt-edged chance following Manuel Akanji's error.
The City goalkeeper denied the onrushing Spurs forward when one-on-one with the visitors 1-0 up, and Postecoglou acknowledged his side failed to seize their opportunities.
"We lost the game so it was a missed chance for three points," the frustrated Tottenham manager told Sky Sports.
"We had the opportunities and we had to punish them. It was a tight game and in the big moments we were not able to capitalise and they were.
"I thought we were in the game the whole time, even at 1-0 we had a great chance to go level.
"In the second half we were chasing the game so they got a little more space but I thought we were well in it up until that point [the second goal]."
Spurs' loss proved Aston Villa's gain as Unai Emery's side qualified for the Champions League for the first time in their history, ending a 41-year absence after last featuring in the European Cup in 1982-83.
Postecoglou found it hard to seek the immediate positives, having watched Tottenham fall short and inadvertently damage rivals Arsenal's title hopes in the process.
"We lost the game so I am disappointed," the Australian added, before responding to whether any pride could be taken.
"Not right now because we have lost. We will assess the season, still one more game to go, one more game against Sheffield United and we need to make sure we win.
"I think wherever you finish is a fair reflection of where you are at."
Tottenham will finish outside the top four for the fourth time in the last five seasons, after finishing in the Champions League spots in Mauricio Pochettino’s final four full campaigns in charge (2015-16 to 2018-19).
Postecoglou acknowledged there is still work to do to catch up with England's perennial champions City.
Asked how his side measure up to Guardiola's men, he said: Nothing earth-shattering that I didn't know before the game. We still have some work to do. We need to do some work to catch up."
Real Madrid 5-0 Alaves: Vinicius double continues LaLiga champions party
Vinicius Junior scored twice as newly-crowned LaLiga champions Real Madrid thrashed visitors Alaves 5-0 on Tuesday, after the hosts had paraded the trophy in front of the home fans before the game.
Jude Bellingham and Vinicius scored a goal each inside 30 minutes before Federico Valverde put Los Blancos 3-0 up at the break, unleashing an unstoppable shot into the roof of the net in first-half stoppage time.
Vinicius added one more in the 70th minute from a precise cross by Bellingham that the Brazilian fired into the top corner, and substitute Arda Guler completed the scoring with a low finish.
Carlo Ancelotti's side travel to Villarreal on Sunday before hosting Real Betis on May 25 ahead of their Champions League final against Borussia Dortmund on June 1.
Having thrashed Granada 4-0 on Saturday and Cadiz 3-0, either side of their Champions League semi-final victory over Bayern, Real are firing on all fronts heading into the Wembley Stadium showpiece.
Meanwhile, Alavez remain 11th in the table heading into their next game against Getafe at home on Saturday.
Data Debrief: Madrid capitalise on opportunities
Madrid found the back of the net five times from just 2.47 expected goals (xG), suggesting Los Blancos' finishing quality was far greater than Alaves' 1.28 xG.
Ancelotti's side have now kept 20 clean sheets in 36 LaLiga games this campaign, their most in a single season in the competition's history (19 in the 1986-87, 1987-88 and 2019-20 seasons).
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