Serena Williams will play in the Emilia-Romagna Open next week following her early exit in Rome, Patrick Mouratoglou has confirmed.

The legendary American was beaten 7-6 (8-6) 7-5 by Nadia Podoroska in the 1,000th match of her incredible career at the Internazionali BNL d'Italia on Wednesday.

Williams, who was given a bye into the second round at Foro Italico, was playing her first match since losing to Naomi Osaka in the semi-finals of the Australia Open three months ago.

With the French Open getting under way on May 30, Williams has taken a wildcard to get more time on clay under her belt in Parma.

Mouratoglou, the former world number one's coach, tweeted: "Change of plans: Serena hasn't been competing for a while, and we want to get as many matches under our belt as possible before Roland-Garros - so we're adding the Emilia-Romagna Open to our schedule.

"We'll be back in action next week."

Williams will head to Paris for the second major of the year in her latest attempt at matching Margaret Court's record tally of 24 grand slam singles titles.

Simona Halep suffered a worrying leg injury just over two weeks out from the French Open at the Internazionali BNL d'Italia, where Serena Williams and Naomi Osaka crashed out.

Halep, the 2018 French Open champion, had dominated the first set against Angelique Kerber, winning 6-1, but came off the court with an apparent calf problem with the scores level at 3-3 in the second.

She received treatment but an emotional Halep was forced to withdraw from the clash, the defending champion handing victory to Kerber.

The Romanian was then helped from the grandstand court, leaving the area with strapping on her leg and a heavy limp, raising doubts about her ability to compete at Roland Garros, where she would likely be one of the favourites.

Earlier Williams made her first appearance on the tour since February, losing the 1,000th match of her glittering career to Nadia Podoroska, who reached the semi-finals in Paris last year.

Podoroska prevailed 7-6 (8-6) 7-5, with 23-time grand slam champion Williams not too downhearted by a defeat to an accomplished clay-court player.

"It's tough to have a first match on clay. It was definitely kind of good to go the distance and to try to be out there, but clearly I can do legions better," Williams said. 

"She has a good game, for sure, obviously. She's very consistent. Overall, it was good for me to play such a clay-court player on clay today, but it's a little frustrating. But it's all right. It is what it is.

"I have been training for months, but it feels definitely different on clay to make that last adjustment. Just filling out the game, finding the rhythm. Even sliding and confidence with that, with movement. That's always like a little struggle in the first two matches, and then I'm raring to go."

Osaka has yet to find her footing on clay and the reigning Australian Open champion came up short in the second round as she suffered a surprise loss to Jessica Pegula.

Pegula said after her 7-6 (7-2) 6-2 win: "I know she doesn't love clay, so I just tried to be the clay court player out there today, and it worked just good enough."

World number one Ashleigh Barty had no such difficulties in her 6-4 6-1 over Yaroslava Shvedova, but fourth seed Sofia Kenin was a shock loser to Barbora Krejcikova.

Petra Kvitova was beaten in three sets by Vera Zvonareva but Madrid champion Aryna Sabalenka, last year's French Open champion Iga Swiatek, Elina Svitolina, Karolina Pliskova and Garbine Muguruza all progressed while teenager Coco Gauff knocked out 17th seed Maria Sakkari.

Serena Williams made an unsuccessful return to the WTA Tour as she joined Naomi Osaka in crashing out of the Internazionali BNL d'Italia in the second round on Wednesday.

Williams was playing the 1,000th match of her glittering career against Nadia Podoroska, her first since losing in the Australian Open semi-finals to Osaka in February.

The American has experienced significant success in Rome, winning the title four times, but was always likely to be tested by a player who reached the French Open semi-finals last year.

And that proved the case as she slumped to a 7-6 (8-6) 7-5 defeat despite threatening a second set fightback.

Having won the first set on a tie-break, Podoroska continued to dominate the longer exchanges and went 5-2 up in the second.

From there, Williams won 12 straight points to level matters at 5-5, but Podoroska then claimed a nervy service hold to force the 23-time grand slam champion to serve to stay in the match.

She never came close to doing so as Williams was broken to love, a miscued forehand long giving Podoroska one of the biggest wins of her career.

Earlier in the day, Osaka was defeated in straight sets by Jessica Pegula.

Osaka has made no secret of her discomfort on clay, having never gone beyond the third round at Roland Garros.

All of her 10 WTA singles titles have come on the hard court and she was undone on the red dirt once more as Pegula prevailed 7-6 (7-2) 6-2.

Garbine Muguruza eased into the second round of the Internazionali BNL d'Italia on Tuesday thanks to a comfortable victory over lucky loser Patricia Maria Tig, though Belinda Bencic was the biggest casualty on a rain-interrupted day.

Spaniard Muguruza was due to face Anastasia Pavlyuchenkova until the Russian withdrew due to an abdominal issue, giving Tig a reprieve and the chance of an upset.

But Muguruza won all but three games in a comprehensive 6-1 6-2 victory that required little more than an hour on court as the former world number one went to 22-6 for the season.

Conditions were not great as drizzle persisted for much of the match, but Muguruza was grateful to progress after a generally testing day.

"It was a weird day," the 12th seed said afterwards. "I woke up [and the weather was] sunny, had an opponent. Then it turned out I had another opponent and it was raining the whole match. We had a little bit of everything!

"But, Rome, it's such a familiar tournament. I played here so many times, I've had nice matches. I love this clay, the red clay. It's familiar to me. I'm just looking forward to getting as many matches as possible."

Bencic will not be joining her in the next round, as she became the highest seed to be eliminated on the day in a 6-3 6-4 defeat to Kristina Mladenovic.

The two played each other under two weeks ago in the first round of the Madrid Open, with Bencic victorious, but Mladenovic got her revenge as she capitalised on her opponent's seven double faults.

Like Tig, Sloane Stephens was also competing as a lucky loser as she came up against compatriot Madison Keys, though the outcome was no different.

Keys claimed only her second win in six meetings with Stephens but had to come from behind as she eventually clinched it 4-6 6-2 7-5 after two-and-a-half hours on court to set up a clash with 15th seed Iga Swiatek.

Elise Mertens, the 14th seed, was also dumped out of the competition, losing out 4-6 6-2 6-3 to Veronika Kudermetova, whom she had beaten in straight sets in their three WTA Tour contests before this one.

Veteran Kazakh Yaroslava Shvedova, ranked 665 in the world, awaits top seed Ashleigh Barty after the 33-year-old showed character to fight back and beat Martina Trevisan 0-6 7-6 (7-4) 7-6 (8-6), while Nadia Podoroska set up a second-round clash with Serena Williams after seeing off Laura Siegemund 2-6 7-6 (7-3) 6-1.

Petra Kvitova recovered from a poor start to beat Magda Linette in the first round of the Internazionali BNL d'Italia on Monday.

Linette took just 31 minutes to win the first set in Rome, but world number 10 Kvitova responded superbly to win 1-6 6-0 6-2.

Two-time grand slam champion Kvitova racked up 16 unforced errors in the first set, yet rallied like the great competitor she has proved to be for so long to ensure she will face Vera Zvonareva - conqueror of Christina McHale - in the second round.

The 11th seed from the Czech Republic said: "The first set I started very badly. I was missing almost everything. On the court... [the ball] was bouncing everywhere as well. A little bit windy. I just had to get used to probably everything.

"The beginning of the second set when finally I held my serve and broke her serve, I think since then it was much, much better."

French Open champion Iga Swiatek advanced when Alison Riske retired at 5-4 down in the first set due to a recurrence of a left foot injury.

Swiatek, who will take on Sloane Stephens or Madison Keys in the second round, made 18 unforced errors but had won four games in a row from 4-1 down when the American was unable to continue. 

Coco Gauff battled past Yulia Putintseva 7-5 4-6 6-4 to set up a meeting with 17th seed Maria Sakkari  - who fended off qualifier Polona Hercog 7-6 (8-6) 3-6 6-2.

Amanda Anisimova will do battle with Elina Svitolina, a winner of this tournament twice, after defeating Wang Qiang 7-5 6-3. Jessie Pegula, Jennifer Brady and Petra Martic were among the other winners on day one.

Viktorija Golubic won the L'Open 35 de Saint-Malo on Sunday after a straight-sets win over Jasmine Paolini.

A finalist in Lyon and Monterrey in March, the world number 84 claimed a second career WTA125K singles title after a 6-1 6-3 triumph in 67 minutes.

Golubic held her nerve at the key moments in a close contest, landing 75 per cent of first serves and saving five of six break points.

Paolini threatened to make more of a contest in the second set after finally breaking, but the Italian dropped serve with her very next game and saw momentum slip away.

It was Golubic's first title since defeating Jennifer Brady at this level at Indian Wells two years ago.

Aryna Sabalenka won the Madrid Open after a 6-0 3-6 6-4 victory over world number one Ashleigh Barty on Saturday.

Sabalenka wrapped up the first set to love in just 25 minutes as an unusually sluggish Barty struggled to find any kind of rhythm to her game.

Barty, who had won 16 consecutive matches on red clay, rallied in the second set to get herself back on level terms.

At 4-4 in the deciding set Sabalenka broke Barty and then held her nerve on serve to secure her 10th WTA Tour career title which will see her climb up to fourth from seventh in the world rankings on Monday.

Barty was on a streak of nine consecutive WTA Tour titles and had not lost to an opponent in the top 10 since the WTA Finals in 2019.

But in-form Sabalenka had not dropped a single set in Madrid in the lead-up to the final and began in imperious fashion.

The 23-year-old from Belarus produced a clinical first set, dropping just four points, as Barty failed to win a single game in the set to record a bagel for the first time in four years.

The pair exchanged breaks in the second set before Australian Barty, 25, finally discovered some momentum to break her opponent again to seal the set.

Sabalenka squandered a one-set lead to lose to Barty in the Stuttgart Open final two weeks ago.

And Barty, also playing in the final of the tournament for the first time, had threatened to break her opponent at 4-3 in the decider.

But this time Sabalenka took her chance when Barty fluffed a drop shot and then double faulted to give her three break points, converting at the first time of asking with a powerful backhand.

Sabalenka then clinically sealed victory with a flawless service game to love, clinching victory when Barty smashed a forehand into the net.

"To be honest, after the final in Stuttgart I was injured, I couldn't move and wanted to withdraw," Sabalenka said in an on-court interview after the win.

"But the recovery was good, in four days I feel better and now I'm the champion. It's been an amazing week."

Viktorija Golubic will take on Jasmine Paolini in the L'Open 35 de Saint-Malo final after both players won their semi-final contests in straight sets on Saturday.

Italian Paolini won 6-3 6-1 against Varvara Gracheva, who was unable to follow up her impressive quarter-final triumph over second seed Rebecca Peterson.

Golubic meanwhile, is the highest-ranked player remaining and the fifth seed eventually prevailed against Harmony Tan.

In a tough contest, Golubic won 7-6 (7-4) 6-3, continuing a strong run of form that also saw her reach WTA finals in Lyon and Monterrey last month.

Aryna Sabalenka will have a shot at redemption when she faces top seed Ash Barty in the Madrid Open final after beating Anastasia Pavlyuchenkova in straight sets on Thursday.

World number seven Sabalenka squandered a one-set lead to lose to Barty in the Stuttgart Open final two weeks ago.

But she will have another crack at winning silverware in the Spanish capital in Saturday's final against Barty, who saw off Paula Badosa in straight sets in the other last-four match.

"I'm looking forward to the final," she said in her on-court interview. "First of all I will have a good rest and do everything I can to prepare myself as good as I can. 

"It'll be really interesting to play another final against Ash. I think it'll be a great battle. I think everyone coming here can see themselves winning the tournament. I'll do my best."

Sabalenka had not dropped a single set in Madrid in the lead-up to the semi-final and that remains the case after cruising past Pavlyuchenkova in a little over an hour.

The fifth seed converted four of her five break points and won 73 per cent of her second-service points.

Pavlyuchenkova, ranked 41st in the world, lost serve in the fourth and eighth games of the opening set and failed to hold on her first two service games in the second set.

Despite breaking Sabalenka in the seventh game, the Belarussian proved far too strong for her opponent and got over the line with a fourth ace of the match to reach her third final of the season.

Ash Barty saw off Paula Badosa in straight sets to reach the final of the Madrid Open, extending her unbeaten streak on clay to 16 matches.

The world number one triumphed 6-4 6-3 on Thursday, taking her record for 2021 to an impressive 25-3.

Badosa is the only player to have beaten Barty since February, but the Spaniard was unable to pull off a repeat of her upset in their quarter-final clash in Charleston last month.

The world number 62 had become the first Spanish player to reach the semi-finals in Madrid and there was little to split the pair through the opening nine games.

However, when serving at 5-4 down to stay in the set, Badosa suddenly came under pressure.

The wildcard saved two set points but gifted her opponent a 1-0 lead with a double fault. A similar situation early in the second put Barty 3-1 up, though she had to save three break points in her next service game.

After escaping trouble on that occasion, the Australian had no further issues on her way to completing the win after one hour and 15 minutes on court.

Standing in her way of a fourth title this season will be either Aryna Sabalenka or Anastasia Pavlyuchenkova; they will meet in the second semi-final in the evening session in the Spanish capital.

The in-form Barty has already been crowned champion at the Yarra Valley Classic this year, as well as events in Miami and Stuttgart.

Ashleigh Barty will face the only woman on the WTA Tour to have beaten her since February after the world number one set up a Madrid Open semi-final with Paula Badosa.

Barty took her winning streak on the red clay to 15, overcoming ninth seed Petra Kvitova 6-1 3-6 6-3 to reach the last four in Madrid for the first time.

Among active players, the Australian's red-clay run is bettered only by Serena Williams (32 matches in 2013-14) and Sara Errani (16 in 2012).

The 2019 French Open champion is 24-3 for the season and has now won her last 10 matches against top-20 opponents and five of the last six with Kvitova.

Kvitova's hopes of claiming a rare win over Barty were boosted when she sent the match to a decider, but Barty took her formidable record in three-setters this year to 11-1.

"It's just always staying in the fight," Barty said of her performance in deciders this season. "Always staying in the hunt and never letting it slide away.

"That certainly doesn't guarantee results, doesn't guarantee success. It guarantees you give yourself the best chance to do what you do and to figure it out and to find your way."

Finding her way on the WTA Tour is 23-year-old Badosa, who moved up to a career-high ranking of 62 after a run to the semi-finals in Charleston last month.

She defeated Barty in straight sets en route to the last four having beaten Belinda Bencic earlier in the tournament.

Badosa got the better of Bencic again on home soil on Wednesday, prevailing 6-4 7-5, the Swiss paying the price for a string of unforced errors at the end of each set.

"I expect a completely different match," Badosa said of the reunion with Barty.

"She's the number one in the world. Now she knows me. Number one in the world, they don't like to make mistakes two times in a row, so for sure I expect a tough match and completely different to Charleston."

Aryna Sabalenka is through to the semi-finals after Elise Mertens retired from their clash with a thigh injury. Sabalenka had held a commanding 6-1 4-0 lead.

She will face Anastasia Pavlyuchenkova, who is into her first WTA 1000 semi in over a decade after recording her fourth top-25 win of the week by defeating Karolina Muchova in straight sets.

At L'Open 35 de Saint-Malo, sixth seed Arantxa Rus and seventh seed Jasmine Paolini each lost in the round of 16 but fifth seed Viktorija Golubic fought back to defeat Greet Minnen in three sets.

Elise Mertens twice came back from the brink to beat Simona Halep and reach a Madrid Open quarter-final against former doubles partner Aryna Sabalenka.

Third seed Halep was the highest-ranked player in action on Wednesday but came unstuck against Mertens despite taking the opener and leading by a break in each of the subsequent sets.

Mertens emerged a 4-6 7-5 7-5 winner after two hours and 34 minutes, defeating Halep for the first time on clay and only the second time on any surface.

There were opportunities for both players throughout and Halep struck first, losing her break lead in the first but recovering on this occasion.

The Romanian, a two-time winner who has also twice lost in the final in Madrid, twice cancelled out a deficit in the second but could not then capitalise on a lead of her own.

Mertens battled back and broke to love to level the match, then maintained her composure to see out a back-and-forth decider once Halep had again forged ahead.

"She played really well, and she definitely deserved to win because she was stronger in the end," Halep said.

"I cannot say I played bad, but I did some mistakes, important mistakes. Sometimes it's just a little bit and the match is going away."

Sabalenka is up next for Mertens, although she had a much more straightforward path through the third round.

Jessica Pegula held her up for only 52 minutes in a dominant 6-1 6-2 success.

In the same half of the draw, Anastasia Pavlyuchenkova upset another seed, following up her straight-sets victory over Karolina Pliskova by beating Jennifer Brady in three. She plays Karolina Muchova next.

At L'Open 35 de Saint-Melo, second seed Rebecca Peterson came from behind to see off Xiyu Wang, while Tamara Zidansek and Arantxa Rus also produced comebacks.

Nina Stojanovic survived three sets, but Christina McHale crashed out to Viktoriya Tomova.

Ash Barty extended her winning streak to 14 matches on clay with a straight-sets defeat of Iga Swiatek at the Madrid Open.

The 2019 French Open champion defeated last year's winner at Roland Garros 7-5 6-4 to reach the quarter-finals in the Spanish capital.

Swiatek herself came into the contest having won 18 consecutive sets on clay and took early control of the contest as she went 3-0 up in the opener.

However, Barty recovered thanks to imperious play behind her serve as she won 80 per cent of all points behind her first delivery and avoided being broken for the remainder of the contest.

The world number one was 0-40 down at 3-2 in the second but rallied to hold before seeing out the win.

Barty will now face ninth seed Petra Kvitova in the last eight after she battled past Veronika Kudermetova 6-3 4-6 6-4.

The three-time Madrid Open champion required just over two hours to beat the Russian, who has won more matches in 2021 (22) than anyone except Barty (23).

"Always playing Ash, it's a great challenge," Kvitova said. "It will be a great match-up. I really will enjoy it. I'm really looking forward for it.

"She has a game for clay. She's sliding. She's playing lots of slice and everything... She likes clay a little bit more than me probably. I like Madrid. So, who knows, right?"

Eighth seed Belinda Bencic is also through after Ons Jabeur retired from their match with a thigh injury. She will now face Paula Badosa, who needed more than two and a half hours to defeat Anastasija Sevastova in three sets.

At the L'Open 35 de Saint-Malo, Alize Cornet suffered a surprise defeat in the first round as Oceane Dodin prevailed in a marathon match.

Dodin knocked out the top seed in two hours and 56 minutes, recovering after letting two match points slip in the second-set tie-break to win 7-5 6-7 (8-10) 6-1.

Third seed Alison van Uytvanck also made an early exit as Anna Karolina Schmiedlova came from a set down to prevail 2-6 7-5 6-3.

But Viktorija Golubic was victorious, the fifth seed winning in straight sets against Clara Burel.

Naomi Osaka suffered a shock second-round exit to Karolina Muchova at the Madrid Open, but there was no such trouble for Simona Halep as she held off Zheng Saisai.

Second seed Osaka was playing just her second match on clay since the 2019 French Open due to injury issues and became the latest big name to fall at the hands of Muchova.

The world number 20, who had already defeated two top-five players this season, prevailed 6-4 3-6 6-1 in a time of one hour and 49 minutes in the Spanish capital.

Osaka has now lost two of her last three matches, having been eliminated from the Miami Open quarter-finals by Maria Sakkari at the end of March.

"I think today for me it was quite different from the last time I lost in Miami, and I actually think I played much better here, so I'm very happy about that," Osaka said.

"I think what I can take away from this is that I tried my best throughout the entire match. I think tennis-wise, I felt I was too defensive in the first set.

"I wouldn't say I’m happy that I lost, but I think I learned a lot. So that's all I can hope for."

Two-time champion Halep has yet to drop a set in this year's tournament after seeing off unseeded opponent Zheng 6-0 6-4.

Halep took nine games in a row at the start of the match but, like in her opening-round win over Sara Sorribes Tormo, she had to hold off a late surge from her opponent to advance.

Next up for Halep is a last-16 showdown with Elise Mertens, who beat Elena Rybakina 7-6 (7-4) 7-5 in the last of Sunday's matches.

Fifth seed Aryna Sabalenka safely advanced earlier in the day thanks to a 6-3 6-3 win against Daria Kasatkina, while Sakkari beat Anett Kontaveit 6-3 6-1.

Sabalenka was on course to face Victoria Azarenka for a place in the quarter-finals, but the Belarussian withdrew from her match with Jessica Pegula because of a back injury.

Elsewhere, Jennifer Brady, who knocked out fellow American Venus Williams in the first round, eased past Jelena Ostapenko 6-2 6-1.

Brady faces a tricky test in the next round with Anastasia Pavlyuchenkova, who defeated sixth seed Karolina Pliskova 6-0 7-5, seeking another scalp.

Ash Barty overcame Tamara Zidansek and her own disjointed performance to reach the third round of the Madrid Open on Saturday.

The Australian triumphed 6-4 1-6 6-3 after nearly two hours on court to extend her winning streak on clay to 13 matches.

Barty will face reigning French Open champion Iga Swiatek in the next round, the 19-year-old having eased past Laura Siegemund 6-3 6-3 in a contest where she failed to take 10 match points before getting over the line.

Barty endured a more troubled contest with Zidansek, landing just 51 per cent of her first serves.

Zidansek lost the opener after a wayward forehand at 4-4 but was authoritative in the second as she won a set against a top-10 player for the first time.

However, a series of errors early in the decider allowed world number one Barty to open up a lead she did not relinquish. Barty was champion at Roland Garros in 2019 but skipped last year's Paris grand slam, so the clash with Swiatek will pit together the two most recent French Open winners.

"[It was] a really tough match," Barty said after edging out Zidansek. "I felt my margins were a little bit off. I made a lot of errors. I think I was missing in the right way, but still just a foot or two here or there makes a big difference. I felt like there were probably too many loose ones for me today.

"I don't think there was a lot in it by any means. We had a lot of close games in the first set, early in the second set. In the third, as well, there were a lot of close games. I think the response early in the third was good. I was a bit more aggressive, was able to bring the match back on my terms."

Petra Kvitova set up a meeting with Veronika Kudermetova – who knocked out defending champion Kiki Bertens – after overcoming long-time rival Angelique Kerber 6-4 7-5.

Kerber got the opening break in each set, only to drop serve in the next game, with Kvitova ultimately rewarded for powerful hitting as she ended the contest with 33 winners to her opponent's 14.

"I served very well, especially in the important points," said Kvitova, who leads the head-to-head record 8-6. "I think we really played very well. She even served very well, as well. It was tough to attack her serve."

Fifteenth seed Johanna Konta suffered a straight-sets loss to Anastasija Sevastova, while Ons Jabeur fought back to beat Sloane Stephens.