Emma Raducanu retired from her first-round match with Bianca Andreescu at the Internazionali d'Italia because of a lower back injury.
The world number 12 was trailing 6-2 2-1 when calling time on the match against Andreescu in a highly anticipated showdown between two of the past three US Open winners.
Raducanu initially left the court for treatment ahead of the seventh game of the opening set for a medical timeout and completed just four more games.
Speaking after her withdrawal, coming two weeks before the French Open, Raducanu explained she did not want to aggravate an injury sustained at last week's Madrid Open.
"I thought maybe taking one, two days off, it would go away because a lot of the other small niggles I've had, they've kind of gone away after taking, like, two days off," she said.
"Then I got here and I was training, but it just didn't seem to get better. I was training with some limitations. I wasn't moving really.
"I was just playing where I knew where the ball was coming, just staying in one corner. I think I must have underestimated the unpredictability of competition in a match.
"The last few weeks have been really positive. I've learned a lot about myself and my game has definitely improved on this surface but I need to make sure my back is fully right.
"I need to just keep on it. I don't want to play my next match with a feeling of limitation because I think that I learned my lesson from this week, when to push, when not to push."
Ons Jabeur joined Andreescu in the last 32 with a 6-0 7-6 (7-1) victory over Sorana Cirstea to keep her impressive run of form going.
The Tunisian won her first WTA 1000 title in Madrid last week and has now won seven successive main draw matches for the first time in her career
Elsewhere in Tuesday's action, American qualifier Lauren Davis pulled off a shock 6-2 6-3 win against number 11 seed Jelena Ostapenko.
Fellow Americans Jessica Pegula, Madison Brengle and Amanda Anisimova also advanced, beating Liudmila Samsonova, Marta Kostyuk and Tereza Martincova respectively.
Anisimova, who is now the player with the most wins in three sets so far in 2022 with nine, will face Belinda Bencic for a place in the last 16.
Raducanu stunned by qualifier Carle in Madrid
Maria Lourdes Carle made a splash at the Madrid Open on Wednesday, as the qualifier sent Emma Raducanu packing.
World number 82 Carle stepped into the void to fill in for Karolina Pliskova, who withdrew prior to the end of the qualifying rounds, to face 2021 US Open champion Raducanu.
It is just the second time Carle has reached the main draw of a WTA 1000 event, and she ensured it would be a memorable campaign after a 6-2 6-2 victory over Raducanu, who is now ranked at world number 221, though has enjoyed something of a return to form this season.
Raducanu reached the quarter-finals of the Stuttgart Grand Prix last week, going down to world number one Iga Swiatek, but has recently enjoyed victories over the likes of Angelique Kerber and Caroline Garcia.
Data Debrief
Carle is the first player from Argentina to defeat a former women's singles grand slam Champion since Nadia Podoroska overcame Serena Williams in Rome in 2021.
The 24-year-old is making just her sixth WTA main draw appearance, with her first WTA 1000 appearance having come in Miami in March.
'I will play Roland Garros if I feel competitive' – Nadal uncertain on French Open participation
Rafael Nadal is unsure as to whether he will be fit enough to feature at the French Open.
Nadal is embarking on a swansong season, having indicated he will retire after the 2024 campaign.
The 37-year-old is set to make his farewell appearance at the Madrid Open this week, with his campaign starting against American teenager Darwin Blanch on Thursday.
Nadal, now ranked 512 in the world, has played just five Tour-level matches this year, as he makes his comeback from yet another injury lay-off.
And the 14-time Roland Garros champion acknowledged on Wednesday that he would be unlikely to play at the French Open should it be taking place this week.
"If I was in Paris today, I wouldn't go out to play," he said.
"I don't think I'll be able to play at 100 per cent, but it's important to be able to play for the last time in Madrid.
"It means a lot to me to play on this court where I've had some great moments."
Thankfully for Nadal, the French Open does not take place until late May, giving him time to get fit.
"If I arrive in Paris the way I feel today, I will not play," he said.
"I will play Roland Garros if I feel competitive. If I can play, I play. If I can't play, I can't. It won't be the end of the world or the end of my career. I still have goals after Roland Garros, like the Olympics.
"Few weeks [ago], I didn't know if I would be able to play again on the professional tour. It's not perfect but at least I am playing and I can enjoy again, especially in the tournaments that are so emotional for me.
"I'm able to enjoy the fun that I can say, probably, goodbye on court. Without trying to confuse anyone, I don't know what's going to happen in the next three weeks.
"I'm going to do the things I have to do to be able to play in Paris. And if I can, I can and if I can't, I can't. I'm going to Paris if I feel like I'm good enough. I'm going to Paris if I feel capable enough to compete."
Nadal played three times in Brisbane in January, losing to qualifier Jordan Thompson and subsequently missing the Australian Open.
He featured twice in Barcelona earlier in April, losing to Alex De Minaur in the round of 32.
Nadal has won five titles in Madrid, though, and the tournament holds a special place for him, as he underlined his determination to compete.
He added: "Some moments I find myself enjoying being on court, playing against the best players again and I feel myself, more or less competitive, and other moments I feel limitations and it’s difficult.
"The goal is to be on court, enjoy it as long as possible. I mean, that's the thing, enjoy the fact that I will be able to compete one more time on the professional tour and here at home in Madrid, a place that gives me everything in terms of support.
"I am here giving myself a chance. If at some moment my situation improves, if I am able to find better feelings in my body, I need to be ready."
Liverpool line up Feyenoord coach Slot as Klopp replacement
Arne Slot appears to be at the front of the queue to replace Jurgen Klopp as Liverpool manager.
Klopp confirmed in January that he would be leaving the Reds at the end of the season.
Xabi Alonso, who has led Bayer Leverkusen to their first Bundesliga title, was swiftly established as a leading candidate to take the helm at Anfield, where he starred as a player for the Reds between 2004 and 2009.
The 42-year-old, however, is staying put in Germany, and Liverpool's search for Klopp's successor has taken them to Feyenoord's door.
Slot has been previously linked to jobs in the Premier League. Leeds United wanted to appoint him last season before their relegation to the Championship, while he was targeted by Tottenham before they appointed Ange Postecoglou.
The Dutchman intimated when linked with Spurs: "A normal next step would be to go abroad and I've always said that the best league in the world is the Premier League."
Ideally, Liverpool will be aiming for a coach who can come close to replicating Klopp's style of play, and in particular has a flair for working with and developing young players.
Slot has enjoyed fantastic success in Rotterdam. He became Feyenoord head coach in December 2022, after leaving AZ, and went on to win the Eredivisie title in 2022-23, having reached the Europa League final in the previous season.
The 45-year-old has a 64.4 per cent win record from 146 games in charge, winning 94, drawing 29 and losing just 23.
His Feyenoord team have scored 329 goals, an average of 2.2 per match, conceding 147 in return. Slot's points per game is a hugely impressive 2.13.
Feyenoord are, though, set to relinquish their Eredivisie crown, as they trail leaders PSV by nine points. They have, however, won the KNVB Cup this term.
As there is no break clause in the Dutchman's contract, it has been reported that a compensation fee in the region of €10million (£8.5m) could prise him away from Feyenoord.
Sporting CP boss Ruben Amorim is another coach who has been heavily linked with the Liverpool job, albeit he is reportedly in talks with West Ham.
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