Simona Halep suffered a worrying leg injury just over two weeks out from the French Open at the Internazionali BNL d'Italia, where Serena Williams and Naomi Osaka crashed out.
Halep, the 2018 French Open champion, had dominated the first set against Angelique Kerber, winning 6-1, but came off the court with an apparent calf problem with the scores level at 3-3 in the second.
She received treatment but an emotional Halep was forced to withdraw from the clash, the defending champion handing victory to Kerber.
The Romanian was then helped from the grandstand court, leaving the area with strapping on her leg and a heavy limp, raising doubts about her ability to compete at Roland Garros, where she would likely be one of the favourites.
Earlier Williams made her first appearance on the tour since February, losing the 1,000th match of her glittering career to Nadia Podoroska, who reached the semi-finals in Paris last year.
Podoroska prevailed 7-6 (8-6) 7-5, with 23-time grand slam champion Williams not too downhearted by a defeat to an accomplished clay-court player.
"It's tough to have a first match on clay. It was definitely kind of good to go the distance and to try to be out there, but clearly I can do legions better," Williams said.
"She has a good game, for sure, obviously. She's very consistent. Overall, it was good for me to play such a clay-court player on clay today, but it's a little frustrating. But it's all right. It is what it is.
"I have been training for months, but it feels definitely different on clay to make that last adjustment. Just filling out the game, finding the rhythm. Even sliding and confidence with that, with movement. That's always like a little struggle in the first two matches, and then I'm raring to go."
Osaka has yet to find her footing on clay and the reigning Australian Open champion came up short in the second round as she suffered a surprise loss to Jessica Pegula.
Pegula said after her 7-6 (7-2) 6-2 win: "I know she doesn't love clay, so I just tried to be the clay court player out there today, and it worked just good enough."
World number one Ashleigh Barty had no such difficulties in her 6-4 6-1 over Yaroslava Shvedova, but fourth seed Sofia Kenin was a shock loser to Barbora Krejcikova.
Petra Kvitova was beaten in three sets by Vera Zvonareva but Madrid champion Aryna Sabalenka, last year's French Open champion Iga Swiatek, Elina Svitolina, Karolina Pliskova and Garbine Muguruza all progressed while teenager Coco Gauff knocked out 17th seed Maria Sakkari.
Emma Raducanu’s run at Stuttgart Open ended by world number one Iga Swiatek
Emma Raducanu’s progress in the Stuttgart Open was halted in straight sets by world number one Iga Swiatek.
The Polish four-time grand slam champion, in her 100th week on top of the ATP world rankings, prevailed 7-6 (2) 6-3 to set up a semi-final showdown with Kazakhstan’s Elena Rybakina.
It was, however, an encouraging quarter-final performance from Raducanu, who has slipped to 303 in the world rankings after a torrid 2023.
Raducanu came in to the contest on a high after winning four matches in a row for the first time since her US Open title in 2021 and raised confidence levels were reflected in the opening exchanges.
Several booming returns from the 21-year-old Briton turned a 0-40 deficit into an immediate break of serve, but Swiatek levelled after edging a second game that featured seven deuces.
That see-saw battle set the tone for a marathon 70-minute opening set which produced plenty of high-quality ground strokes from both sides of the net.
The match went with serve from that point, with Raducanu superbly holding her nerve at 5-4 and 6-5 down to force the first set to a tie-break.
Swiatek completely dominated the tie-break though, winning the first four points before closing it out 7-2.
Raducanu was quickly in trouble again in the second set, falling 2-0 behind and covered in clay after losing her footing on the baseline.
She continued to fight and fended off break points in her next two service games to stay in the match.
But Swiatek kept up the pressure to finally end Raducanu’s resistance to move in to the last four.
Emma Raducanu promises more after reaching Stuttgart Open quarter-finals
Emma Raducanu says the best is yet to come as she continued her excellent form by making it through to the quarter-final of the Stuttgart Open.
The 2021 US Open champion beat Linda Noskova 6-0 7-5 in Germany to set up a mouth-watering last-eight tie with world number one Iga Swiatek on Friday.
Raducanu is making her way back after a torrid 2023 but looked excellent in winning her two ties in the Billie Jean King Cup at the weekend and then followed that up by swatting home favourite Angelique Kerber aside in Wednesday’s first round.
She stepped it up again to dispose of the world number 31 to set up a repeat of the 2022 quarter-final with Swiatek.
Despite all her troubles since winning the US Open in 2021, Raducanu never doubted the good times would return.
“I am not too surprised because I have been working really hard on the training court and I knew it was just a matter of time,” she said in her on-court interview.
“This isn’t my best I still have a long way to go. I am just really happy the rewards are starting to come on the match court.
“You know how it is when you are training day in, day out you feel like the results aren’t going your way. It is never that far away, that is something I am learning.
“Going through everything I have been through gives you a different appreciation, there is nothing like playing in front of fans and playing good tennis.”
Her progress will face its toughest test when against the Polish world number one, who is a master of the clay court, but the Briton knows she is not under any pressure.
Raducanu, whose world ranking of 303 will shoot up, added: “We played here two years ago in the quarters, I have come a long way in two years, and she has achieved a lot, I have nothing to lose, I can swing and enjoy it.
“Hopefully recover in time. I’m good.”
Emma Raducanu records impressive victory over Angelique Kerber in Stuttgart
Emma Raducanu claimed an emphatic 6-2 6-1 win over former two-time champion Angelique Kerber in the last 32 of the Porsche Grand Prix in Stuttgart.
Fresh from helping Great Britain reach the finals of the Billie Jean Cup at the weekend, Raducanu continued her promising form on clay by breezing past the home favourite.
Both Raducanu and Kerber were playing as wild cards in Stuttgart, with the Briton tumbling down the rankings after time out to undergo three surgeries.
Meanwhile former world number one Kerber only recently returned from 18 months out on maternity leave.
Raducanu got off to a flying start by breaking Kerber in the opening game and despite an immediate response from the German, the 2021 US Open champion took control and claimed the first set in 41 minutes.
Kerber was broken again at the start of the second set and although she managed to haul back level, Raducanu stepped up a gear and shrugged off a brief visit from the trainer to wrap up an emphatic win.
Also in Stuttgart, reigning US Open champion Coco Gauff rallied from 4-2 down in the deciding set to overcome fellow American and world number 134 Sachia Vickery 6-3 4-6 7-5.
And Tunisia’s Ons Jabeur finally snapped a five-game losing streak to beat Ekaterina Alexandrova 2-6 6-3 7-6 (1), telling reporters: “I thought about withdrawing so many times because I couldn’t take another loss.”
In Rouen, Naomi Osaka’s return to clay was cut short by a 6-4 6-2 first round defeat to Martina Trevisan.
Like Kerber, Osaka only recently returned to the tour following maternity leave, and was competing as a wild card.
In the Oeiras Ladies Open in Portugal, Britain’s fifth-seeded Harriet Dart suffered a 6-3 6-1 defeat to home player Matilde Jorge.
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