Henry Slade has withdrawn from the England squad for their Six Nations opener against Scotland on February 4.
New head coach Steve Borthwick confirmed his 36-man selection for the Calcutta Cup on Sunday, with Slade left out after suffering a hip injury in Exeter Chief's European Champions Cup game against Castres last week.
Jamie George has overcome a head injury to make the squad, as has Anthony Watson (leg) after he featured for Leicester Tigers against Northampton Saints on Saturday.
After confirming the squad, Borthwick said: "This is a very important week for us as we prepare for our first game against Scotland.
"We know that Scotland are an excellent side with a great coaching team led by Gregor Townsend. They're a settled squad who have been together a long time and have dominated this fixture in recent seasons.
"They will be coming to Twickenham full of confidence.
"We know we have a lot of work to do. The team trained very hard last week and are ready to go again this week. We can't wait to run out at Twickenham again in front of our incredible supporters."
It was also confirmed that Slade and vice-captain Courtney Lawes will continue rehab work within the camp during the week.
England squad to face Scotland
Forwards: Ollie Chessum, Dan Cole, Ben Curry, Alex Dombrandt, Tom Dunn, Ben Earl, Ellis Genge, Jamie George, Joe Heyes, Jonny Hill, Nick Isiekwe, Maro Itoje, Lewis Ludlam, David Ribbans, Bevan Rodd, Sam Simmonds, Kyle Sinckler, Mako Vunipola, Jack Walker, Jack Willis
Backs: Owen Farrell, Tommy Freeman, Ollie Hassell-Collins, Dan Kelly, Ollie Lawrence, Max Malins, Joe Marchant, Alex Mitchell, Cadan Murley, Fin Smith, Marcus Smith, Freddie Steward, Manu Tuilagi, Jack van Poortvliet, Anthony Watson, Ben Youngs